Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant.
Identifieur interne : 000D49 ( Main/Exploration ); précédent : 000D48; suivant : 000D50Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant.
Auteurs : Ruichao Li [République populaire de Chine] ; Miaomiao Xie [République populaire de Chine] ; Jinfei Zhang [République populaire de Chine] ; Zhiqiang Yang [République populaire de Chine] ; Lizhang Liu [République populaire de Chine] ; Xiaobo Liu [République populaire de Chine] ; Zhiwei Zheng [République populaire de Chine] ; Edward Wai-Chi Chan ; Sheng Chen [Hong Kong]Source :
- The Journal of antimicrobial chemotherapy [ 1460-2091 ] ; 2017.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Animaux (MeSH), Antibactériens (pharmacologie), Biologie informatique (MeSH), Chine (MeSH), Colistine (pharmacologie), Escherichia coli (effets des médicaments et des substances chimiques), Escherichia coli (génétique), Fermes (MeSH), Gènes bactériens (MeSH), Plasmides (classification), Protéines Escherichia coli (génétique), Résistance bactérienne aux médicaments (MeSH), Suidae (microbiologie), Séquences répétées dispersées (MeSH), Tests de sensibilité microbienne (MeSH), Transfert horizontal de gène (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Escherichia coli.
- génétique : Escherichia coli, Plasmides, Protéines Escherichia coli.
- microbiologie : Suidae.
- pharmacologie : Antibactériens, Colistine.
- Analyse de séquence d'ADN, Animaux, Biologie informatique, Chine, Fermes, Gènes bactériens, Résistance bactérienne aux médicaments, Séquences répétées dispersées, Tests de sensibilité microbienne, Transfert horizontal de gène.
English descriptors
- KwdEn :
- Animals (MeSH), Anti-Bacterial Agents (pharmacology), China (MeSH), Colistin (pharmacology), Computational Biology (MeSH), Drug Resistance, Bacterial (MeSH), Escherichia coli (drug effects), Escherichia coli (genetics), Escherichia coli Proteins (genetics), Farms (MeSH), Gene Transfer, Horizontal (MeSH), Genes, Bacterial (MeSH), Interspersed Repetitive Sequences (MeSH), Microbial Sensitivity Tests (MeSH), Plasmids (classification), Sequence Analysis, DNA (MeSH), Swine (microbiology).
- MESH :
- chemical , genetics : Escherichia coli Proteins.
- chemical , pharmacology : Anti-Bacterial Agents, Colistin.
- classification : Plasmids.
- drug effects : Escherichia coli.
- genetics : Escherichia coli.
- microbiology : Swine.
- Animals, China, Computational Biology, Drug Resistance, Bacterial, Farms, Gene Transfer, Horizontal, Genes, Bacterial, Interspersed Repetitive Sequences, Microbial Sensitivity Tests, Sequence Analysis, DNA.
Abstract
OBJECTIVES
To analyse and compare mcr-1-bearing plasmids from animal Escherichia coli isolates, and to investigate potential mechanisms underlying dissemination of mcr-1.
METHODS
Ninety-seven ESBL-producing E. coli strains isolated from pig farms in China were screened for the mcr-1 gene. Fifteen mcr-1-positive strains were subjected to molecular characterization and bioinformatic analysis of the mcr-1-bearing plasmids that they harboured.
RESULTS
Three major types of mcr-1-bearing plasmids were recovered: IncX4 (∼33 kb), IncI2 (∼60 kb) and IncHI2 (∼216-280 kb), among which the IncX4 and IncI2 plasmids were found to harbour the mcr-1 gene only, whereas multiple resistance elements including bla
CONCLUSIONS
The mcr-1 gene can be disseminated via multiple mobile elements including Tn6330, its circular intermediate and plasmids harbouring such elements. It is often co-transmitted with other resistance determinants through IncHI2 plasmids. The functional mechanism of Tn6330, a typical composite transposon harbouring mcr-1, should be further investigated.
DOI: 10.1093/jac/dkw411
PubMed: 28073961
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Anti-Bacterial Agents (pharmacology)</term>
<term>China (MeSH)</term>
<term>Colistin (pharmacology)</term>
<term>Computational Biology (MeSH)</term>
<term>Drug Resistance, Bacterial (MeSH)</term>
<term>Escherichia coli (drug effects)</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli Proteins (genetics)</term>
<term>Farms (MeSH)</term>
<term>Gene Transfer, Horizontal (MeSH)</term>
<term>Genes, Bacterial (MeSH)</term>
<term>Interspersed Repetitive Sequences (MeSH)</term>
<term>Microbial Sensitivity Tests (MeSH)</term>
<term>Plasmids (classification)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Swine (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Antibactériens (pharmacologie)</term>
<term>Biologie informatique (MeSH)</term>
<term>Chine (MeSH)</term>
<term>Colistine (pharmacologie)</term>
<term>Escherichia coli (effets des médicaments et des substances chimiques)</term>
<term>Escherichia coli (génétique)</term>
<term>Fermes (MeSH)</term>
<term>Gènes bactériens (MeSH)</term>
<term>Plasmides (classification)</term>
<term>Protéines Escherichia coli (génétique)</term>
<term>Résistance bactérienne aux médicaments (MeSH)</term>
<term>Suidae (microbiologie)</term>
<term>Séquences répétées dispersées (MeSH)</term>
<term>Tests de sensibilité microbienne (MeSH)</term>
<term>Transfert horizontal de gène (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Escherichia coli Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term>
<term>Colistin</term>
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<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Plasmids</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term>
<term>Plasmides</term>
<term>Protéines Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Suidae</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Swine</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term>
<term>Colistine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>China</term>
<term>Computational Biology</term>
<term>Drug Resistance, Bacterial</term>
<term>Farms</term>
<term>Gene Transfer, Horizontal</term>
<term>Genes, Bacterial</term>
<term>Interspersed Repetitive Sequences</term>
<term>Microbial Sensitivity Tests</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Biologie informatique</term>
<term>Chine</term>
<term>Fermes</term>
<term>Gènes bactériens</term>
<term>Résistance bactérienne aux médicaments</term>
<term>Séquences répétées dispersées</term>
<term>Tests de sensibilité microbienne</term>
<term>Transfert horizontal de gène</term>
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<front><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b>
</p>
<p>To analyse and compare mcr-1-bearing plasmids from animal Escherichia coli isolates, and to investigate potential mechanisms underlying dissemination of mcr-1.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>Ninety-seven ESBL-producing E. coli strains isolated from pig farms in China were screened for the mcr-1 gene. Fifteen mcr-1-positive strains were subjected to molecular characterization and bioinformatic analysis of the mcr-1-bearing plasmids that they harboured.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Three major types of mcr-1-bearing plasmids were recovered: IncX4 (∼33 kb), IncI2 (∼60 kb) and IncHI2 (∼216-280 kb), among which the IncX4 and IncI2 plasmids were found to harbour the mcr-1 gene only, whereas multiple resistance elements including bla</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The mcr-1 gene can be disseminated via multiple mobile elements including Tn6330, its circular intermediate and plasmids harbouring such elements. It is often co-transmitted with other resistance determinants through IncHI2 plasmids. The functional mechanism of Tn6330, a typical composite transposon harbouring mcr-1, should be further investigated.</p>
</div>
</front>
</TEI>
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<DateCompleted><Year>2017</Year>
<Month>08</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>12</Month>
<Day>14</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2091</ISSN>
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<Issue>2</Issue>
<PubDate><Year>2017</Year>
<Month>02</Month>
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<Title>The Journal of antimicrobial chemotherapy</Title>
<ISOAbbreviation>J Antimicrob Chemother</ISOAbbreviation>
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<ArticleTitle>Genetic characterization of mcr-1-bearing plasmids to depict molecular mechanisms underlying dissemination of the colistin resistance determinant.</ArticleTitle>
<Pagination><MedlinePgn>393-401</MedlinePgn>
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<ELocationID EIdType="doi" ValidYN="Y">10.1093/jac/dkw411</ELocationID>
<Abstract><AbstractText Label="OBJECTIVES">To analyse and compare mcr-1-bearing plasmids from animal Escherichia coli isolates, and to investigate potential mechanisms underlying dissemination of mcr-1.</AbstractText>
<AbstractText Label="METHODS">Ninety-seven ESBL-producing E. coli strains isolated from pig farms in China were screened for the mcr-1 gene. Fifteen mcr-1-positive strains were subjected to molecular characterization and bioinformatic analysis of the mcr-1-bearing plasmids that they harboured.</AbstractText>
<AbstractText Label="RESULTS">Three major types of mcr-1-bearing plasmids were recovered: IncX4 (∼33 kb), IncI2 (∼60 kb) and IncHI2 (∼216-280 kb), among which the IncX4 and IncI2 plasmids were found to harbour the mcr-1 gene only, whereas multiple resistance elements including bla<sub>CTX-M</sub>
, bla<sub>CMY</sub>
, bla<sub>TEM</sub>
, fosA, qnrS, floR and oqxAB were detected, in various combinations, alongside mcr-1 in the IncHI2 plasmids. The profiles of mcr-1-bearing plasmids in the test strains were highly variable, with coexistence of two mcr-1-bearing plasmids being common. However, the MIC of colistin was not affected by the number of mcr-1-carrying plasmids harboured. Comparative analysis of the plasmids showed that they contained an mcr-1 gene cassette with varied structures (mcr-1-orf, ISApl1-mcr-1-orf and Tn6330), with the IncHI2 type being the most active in acquiring foreign resistance genes. A novel transposon, Tn6330, with the structure ISApl1-mcr-1-orf-ISApl1 was found to be the key element mediating translocation of mcr-1 into various plasmid backbones through formation of a circular intermediate.</AbstractText>
<AbstractText Label="CONCLUSIONS">The mcr-1 gene can be disseminated via multiple mobile elements including Tn6330, its circular intermediate and plasmids harbouring such elements. It is often co-transmitted with other resistance determinants through IncHI2 plasmids. The functional mechanism of Tn6330, a typical composite transposon harbouring mcr-1, should be further investigated.</AbstractText>
<CopyrightInformation>© The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Ruichao</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xie</LastName>
<ForeName>Miaomiao</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Jinfei</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Zhiqiang</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Lizhang</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Xiaobo</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Zhiwei</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chan</LastName>
<ForeName>Edward Wai-Chi</ForeName>
<Initials>EW</Initials>
<AffiliationInfo><Affiliation>The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Sheng</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, P. R. China sheng.chen@polyu.edu.hk.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>09</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Antimicrob Chemother</MedlineTA>
<NlmUniqueID>7513617</NlmUniqueID>
<ISSNLinking>0305-7453</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029968">Escherichia coli Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C000614037">MCR-1 protein, E coli</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>Z67X93HJG1</RegistryNumber>
<NameOfSubstance UI="D003091">Colistin</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N">China</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003091" MajorTopicYN="N">Colistin</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D024881" MajorTopicYN="Y">Drug Resistance, Bacterial</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029968" MajorTopicYN="N">Escherichia coli Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000072480" MajorTopicYN="N">Farms</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D022761" MajorTopicYN="N">Gene Transfer, Horizontal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005798" MajorTopicYN="N">Genes, Bacterial</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020071" MajorTopicYN="N">Interspersed Repetitive Sequences</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>05</Month>
<Day>27</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>07</Month>
<Day>25</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>08</Month>
<Day>29</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>1</Month>
<Day>12</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>8</Month>
<Day>18</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>1</Month>
<Day>12</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28073961</ArticleId>
<ArticleId IdType="pii">dkw411</ArticleId>
<ArticleId IdType="doi">10.1093/jac/dkw411</ArticleId>
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</pubmed>
<affiliations><list><country><li>Hong Kong</li>
<li>République populaire de Chine</li>
</country>
<region><li>Guangdong</li>
</region>
<settlement><li>Shenzhen</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Chan, Edward Wai Chi" sort="Chan, Edward Wai Chi" uniqKey="Chan E" first="Edward Wai-Chi" last="Chan">Edward Wai-Chi Chan</name>
</noCountry>
<country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Li, Ruichao" sort="Li, Ruichao" uniqKey="Li R" first="Ruichao" last="Li">Ruichao Li</name>
</region>
<name sortKey="Liu, Lizhang" sort="Liu, Lizhang" uniqKey="Liu L" first="Lizhang" last="Liu">Lizhang Liu</name>
<name sortKey="Liu, Xiaobo" sort="Liu, Xiaobo" uniqKey="Liu X" first="Xiaobo" last="Liu">Xiaobo Liu</name>
<name sortKey="Xie, Miaomiao" sort="Xie, Miaomiao" uniqKey="Xie M" first="Miaomiao" last="Xie">Miaomiao Xie</name>
<name sortKey="Yang, Zhiqiang" sort="Yang, Zhiqiang" uniqKey="Yang Z" first="Zhiqiang" last="Yang">Zhiqiang Yang</name>
<name sortKey="Zhang, Jinfei" sort="Zhang, Jinfei" uniqKey="Zhang J" first="Jinfei" last="Zhang">Jinfei Zhang</name>
<name sortKey="Zheng, Zhiwei" sort="Zheng, Zhiwei" uniqKey="Zheng Z" first="Zhiwei" last="Zheng">Zhiwei Zheng</name>
</country>
<country name="Hong Kong"><noRegion><name sortKey="Chen, Sheng" sort="Chen, Sheng" uniqKey="Chen S" first="Sheng" last="Chen">Sheng Chen</name>
</noRegion>
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